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. 2018 Jun 27;293(34):13191–13203. doi: 10.1074/jbc.RA118.002649

Figure 3.

Figure 3.

Effect of C25-140 on E1 and E2 enzymes. Various E1–E2 ubiquitination reactions, including UbcH13–Uev1a, were carried out in the absence or presence of C25-140 (30 μm). None of the reactions is affected by the compound. C, control without ATP; D, DMSO; 140, compound C25-140.