Figure 10.

Analysis of transcriptional bursting parameters in nuclei following depletion of Snf2h in lens fiber cells shows distinct changes in transcriptional bursting parameters for each gene. A, quantification of transcription burst fraction as of β-actin, αA-, βB1-, and γA-crystallin genes in newborn WT versus Snf2h null mouse lens. The lens tissues in this experiment was not divided into areas a–d, but rather sets of nuclei were randomly chosen from all over the tissue due to the disorganized nature of the Snf2h null lens. Transcription burst fraction shown as percent alleles transcribed within the whole tissue of each gene, β-actin, αA-, βB1-, and γA-crystallin genes. B, transcriptional intensity measured by mean fluorescence intensities of nascent transcription sites of the four indicated genes from the whole newborn mouse lens tissue. * denotes significance with p value ≤0.05; n.s. indicates not significant; a.u. denotes arbitrary units. Numbers of nuclei analyzed are shown in Tables S1 and S2, and standard deviations of these measurements are shown in Tables S3 and S4.