Figure 3.

Nascent transcription sites of various genes at embryonic developmental stage E12.5 show different spatial patterns of transcription for each gene. RNA FISH was performed to show nascent transcription sites of β-actin, αA-, βB1-, and γA-crystallin genes in lens tissue regions a, b, c, and d of E12.5 mouse embryonic stage. The areas analyzed are shown in Fig. 1. Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue. Nuclei were stained by DAPI (blue) and outlined by dotted white lines. Areas c and d are shown as a combination at these earlier embryonic developmental stages because it better represents the future region that will eventually undergo denucleation in later developmental stages. Images of nuclei were magnified and adjusted for brightness/contrast for viewing for this figure. Scale bar, 10 μm.