Figure 5.

Nascent transcription sites of various genes at embryonic developmental stage E16.5 during mouse lens fiber cell differentiation show different spatial patterns of transcription for each gene. RNA FISH was performed to show nascent transcription sites of β-actin, αA-, βB1-, and γA-crystallin genes in lens tissue regions a, b, c, and d of E16.5 mouse embryonic stage. The areas analyzed are shown in Fig. 1. Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue. Nuclei was stained by DAPI (blue) and outlined by dotted white lines. Images of nuclei were magnified and adjusted for brightness/contrast for viewing for this figure. Scale bar, 10 μm.