Figure 8.

Denucleation of lens fiber cells is accompanied by nuclear condensation and transfer of nuclear proteins into the cytoplasm. Shown is immunofluorescence analysis of lamin B1, histone H3, H3K27me3, H2BK12Ac, Brg1, and Snf2h (Smarca5) in early differentiated lens fiber nuclei in region a versus terminally differentiated nuclei about to undergo denucleation in region c. The puncta staining pattern of H3K27me3 are sites of inactivated X-chromosome. Images of nuclei were magnified and adjusted for brightness for viewing for this figure. Scale bar, 10 μm.