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. 2018 Jul 5;293(34):12992–13005. doi: 10.1074/jbc.RA118.004215

Figure 3.

Figure 3.

Y1–3 proteins bind to HIV-1 gRNA in infected HeLa/CD4 cells. A, immunoblotting of Y1–3 in the input and IP samples from HeLa/CD4 cells infected with HIV-1. HeLa/CD4 cells stably overexpressing FLAG-tagged Y1–3 proteins, MAL (MyD88 adapter-like protein), or empty vector control cells were infected with HIV-1NL4-3 at an m.o.i. of 5 for 3 h. At 3 h post-infection, FLAG antibodies were used to immunoprecipitate Y1–3 proteins or MAL in HeLa/CD4 cells. B, HIV-1 gRNA is bound by Y1–3 proteins expressed in HeLa/CD4 cells. HIV-1 infection of HeLa/CD4 cells overexpressing Y1–3 proteins, MAL, or empty vector control cells as described above in A. Cell lysates were immunoprecipitated with anti-FLAG antibody, and HIV-1 gag RNA levels were quantified by RT-qPCR. Dunnett's multiple comparison test was used to determine statistical significance. ***, p < 0.0005 compared with the vector control cells. Data presented are representative of four independent experiments.