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Precipitation assays of SUMO proteases. Data points indicate the concentration of soluble protease remaining after equilibration at room temperature with increasing concentrations of the protein precipitant, PEG 8000. The y intercept of each log-linear trend line is S0, and this value represents the theoretical maximum solubility of the protein in the absence of precipitant. Error bars, S.D. from three or more replicates.
