Table 1.

Summary of solubility-enhancing mutations

Residuea	Ulp1_WT	Ulp1_R1	Ulp1_R2	Ulp1_R3	Ulp1_R4
420	Ala	Asp	Asn	Asp	Glu
444	Ala	Glu	Glu	Glu	Arg
539	Ala	Asp	Asp	Asp	Asp
540	Met	Ser	Asp	Ser	Ser
542	Phe	Lys	Lys	Lys	Lys
543	Ala	Gln	Arg	Gln	Gln
553	Met	Lys	Arg	Glu	Lys
567	Ile	Arg	Arg	Arg	Arg
601	Tyr	Gln	Gln	Ser	Gln
605	Ile	Glu	Glu	Glu	Glu

^a Residues are numbered according to PDB entry 1EUV.