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. 2018 Jun 27;293(34):13125–13133. doi: 10.1074/jbc.RA118.002196

Figure 2.

Figure 2.

SMILE inhibits CRTC2-induced gluconeogenic enzyme gene expression in hepatocytes. A, AML12 cells were cotransfected with PEPCK-Luc, G6Pase-Luc, CRTC2, and SMILE expression vectors. B, primary hepatocytes were infected with Ad-GFP, Ad-CRTC2, and Ad-SMILE for 24 h, and gluconeogenic gene expression was analyzed by quantitative PCR. C, primary hepatocytes were infected with Ad-CRTC2 and Ad-SMILE for 24 h, and glucose output assay was performed using glucose-free media supplemented with gluconeogenic substrate sodium lactate (20 mmol/liter) and sodium pyruvate (1 mmol/liter). D, primary hepatocytes were infected with Ad-CRTC2, Ad-US, or Ad-shSMILE for 24 h and treated with insulin for 9 h. Glucose output assay was performed using glucose-free media supplemented with gluconeogenic substrate sodium lactate (20 mmol/liter) and sodium pyruvate (1 mmol/liter). Error bars show mean ± S.D. *, p < 0.05; ***, p < 0.005 by two-tailed Student's t test. n.s. means not significant. Results are representative of three independently performed experiments.