Figure 5.

CREB/CRTC2-induced increase in blood glucose level is reduced by WT SMILE, but not by leucine zipper–mutated SMILE. A–E, recombinant Ad-GFP, Ad-S171A, Ad-SMILE (WT SMILE), and Ad-L5V (leucine zipper mutant SMILE) were delivered by tail vein injection into C57BL/6 mice (n = 6). A, in vivo experiment scheme. B, expression of G6Pase, Pepck, Pgc-1α, albumin, and SMILE was analyzed by real-time quantitative RT-PCR. Expression was normalized to that of ribosomal L32. C, fasting glucose measured after 16 h of fasting, n = 6. D, glucose tolerance test in mice, glucose (2g/kg), right panel area under curve (AUC), n = 6. E, pyruvate tolerance test in mice, pyruvate (2g/kg), right panel, AUC, n = 6. F, insulin tolerance test (0.75 units/kg) in mice, right panel AUC, n = 6. Statistical test for B–E: One-way ANOVA with Tukey's multiple comparison test. n.s., not significant; **, p < 0.01. Data are represented as mean ± S.E.