Table 1.
Potency of cipargamin against parasite proliferation and PfATP4-associated ATPase activity in two PfATP4-mutant lines and their parental lines
The data presented are the mean IC50 ± S.D. from the number of experiments indicated in parentheses. *, p < 0.05; **, p < 0.01; ***, p < 0.001 (t-tests compared with matched parental line, paired or unpaired as appropriate).
| Cipargamin IC50 (nm) |
||
|---|---|---|
| Parasite proliferation | PfATP4-associated ATPase activity | |
| Parent of Dd2-PfATP4T418N | 0.84 ± 0.17 (n = 6) | 12.3 ± 3.3 (n = 4) |
| Dd2-PfATP4T418N | 4.3 ± 0.5 (n = 6)*** | 21.1 ± 3.0 (n = 5)** |
| Parent of Dd2-PfATP4T418N,P990R | 0.66 ± 0.09 (n = 3) | 13.9 ± 1.4 (n = 3) |
| Dd2-PfATP4T418N,P990R | 10.6 ± 2.2 (n = 3)* | 32.5 ± 3.2 (n = 3)** |