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. 2018 Jul 3;293(34):13112–13124. doi: 10.1074/jbc.RA118.003421

Figure 3.

Figure 3.

Generation of DT40–3KO cells stably expressing various types of fragmented R2 and R3. A–F, tryptic fragmentation sites (Arg-919, Arg-1583, and Arg-1884) and the predicted calpain fragmentation site (Glu-1869) on R2 and tryptic fragmentation sites (Arg-915, Arg-1574, Arg-1856, and Arg-1869) on R3 (A and D). The epitopes for the R2 N-terminal antibody NT2 (aa 320–338), the R2 C-terminal antibody CT2 (aa 2686–2701), the R3 N-terminal antibody NT3 (aa 22–230), and the R3 C-terminal antibody (aa 2658–2670) are labeled. cDNA encoding complementary pairs of polypeptide chains corresponding to various types of fragmented R2 and R3 were stably transfected into DT40–3KO cells. Western blot (WB) assays confirmed the expression of both the N-terminal fragment and the complementary C-terminal fragment of each type of fragmented R2 (B and C) or fragmented R3 (E and F) in DT40–3KO cells.