Figure 4.

Fragmentation of R2 and R3 in T7 Mice. A–D, T7 mice received three injections of saline or caerulein hourly. Pancreata were then removed, homogenized, and prepared for Western blot (WB) detection. Although the ratio of the amount of R2 fragments to R2 full-length was still significantly increased (A and C), knockdown of trypsinogen 7 completely blocked the increase of the ratio for R3 (B and D). The arrows in A indicate the major receptor fragments. *, statistical significance determined by Student's t test; n.s., not significant.