Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Fluorescence titration data used to calculate the dissociation constant for ThDP in WT E1 (A) and the first binding site in αV138M E1 (B). Apo-WT E1 (0.034 mg/ml) or apo-αV138M E1 (0.10 mg/ml) in 20 mm KH2PO4 (pH 7.0) containing 1 mm MgCl2 was titrated with 0.2–100 μm ThDP.
