Figure 5. Inhibition of Autophagy during T Cell Activation Results in Decreased Metabolic Output.

(A and B) OCR (A) and ECAR (B) measured in activated Th1 cells in the absence or presence of 3MA or L/N.

(C and D) IL-2 production (C) and cell proliferation (D) of Th1 cells pre-activated in the presence or absence of oligomycin (Oligo) or 2-deoxyglucose (2DG) for 24 hr, washed, and re-stimulated 5 days later.

(E and F) IL-2 production (E) and cell proliferation (F) of Th1 cells left untreated or treated with oligomycin for 24 hr, washed, and stimulated 5 days later.

Data represent mean + SEM from four (A and B) or three (C–F) different experiments. *p < 0.05; **p < 0.01 (ANOVA with Tukey post-test). See also Figure S5.