Fig. 1.

Overall process of the FRET measurement by SLiM. Confocal images of the plant cell wall sample are acquired (1) and a spectral characterisation is performed to determine optimal acquisition conditions by measuring the autofluorescence of the fluorescent probe to be assayed (2). Correlated spectral and lifetime analysis (3) then allows to determine unambiguous FRET signature while careful lifetime analysis (4) provides a quantitative FRET estimation between the fluorescent probe and the plant cell wall sample