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. 2018 Aug 27;14:74. doi: 10.1186/s13007-018-0342-3

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Evolution of T_m according to wavelength channels analysed for WS, WS + PR10, WS + DR10 and phosphate buffer + PR10. Inset indicates the T_m for channel 2 which is selected for FRET evaluation. Phosphate buffer + PR10 sample did not have any component in channel 2