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. 2001 Nov 6;98(24):13675–13680. doi: 10.1073/pnas.241417098

Figure 1.

Figure 1

The dam1-1 mutation leads to chromosome missegregation at the nonpermissive temperature. Large-budded dam1-1 cells at permissive temperature (25°C) and nonpermissive temperature (37°C). Green shows chromosome V-GFP; blue shows DAPI (DNA). (A) At 25°C, two separated dots in different buds, 95% (n = 206) and at 37°C, two separated dots, 0%, two close dots in the same bud, 32%, one dot, 68% (n = 205). Red shows primary rat anti-α tubulin plus secondary Texas red anti-rat Abs. (Bar = 1 μm.) (B) Pds1p-myc = Red shows primary rabbit anti-Myc plus secondary Cy3-conjugated anti-rabbit Abs. (Bar = 1 μm.) (C) Wild-type and dam1-1 cells at 37°C after nocodazole treatment. Cells with one dot: wild-type = 93% (n = 200); dam1-1 = 95% (n = 200). (Bar = 1.5 μm.)