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. 2001 Nov 6;98(24):13675–13680. doi: 10.1073/pnas.241417098

Figure 4.

Figure 4

Dam1p is required for metaphase spindle assembly and anaphase spindle elongation. Blue shows DAPI (DNA), red shows microtubules detected with rat anti-tubulin Abs plus FITC anti-rat secondary Ab (pseudocolored red), and green (Insets) are Dam1GFPp. Wild-type and dam1-1 cells after induction of GAL-MPS1 and release from α factor in four different conditions. (A) Gal23, galactose medium at 23°C; Gal33, galactose medium at 33°C; and Gal23–33, galactose medium at 23°C then shifted to 33°C. (Bar = 1.5 μm.) (B) Gal23–33-Glu37, galactose medium at 23°C then shifted to 33°C and then shifted to glucose medium at 37°C plus α factor. (Bar = 5 μm.)