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. 2017 Oct;58(12):5412–5420. doi: 10.1167/iovs.17-22325

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Copyright 2017 The Authors

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Immunoblots of caspases, calpains, and their substrates in RPE cells cultured with A2E. (Lane 1) initial, (lane 2) 12-hour normal, (lane 3) 12-hour 25 μM A2E, (lane 4) 1-day normal, (lane 5) 1-day 25 μM A2E, (lane 6) 2-day normal, and (lane 7) 2-day 25 μM A2E. (A) Caspase-3, (B) calpain 1, (C) calpain 2, (D) α-spectrin, and (E) β-actin. Bar graphs show the densities of α-spectrin fragments at (F) 145 kDa (calpain-specific) and at (G) 120-kDa (caspase-3-specific) normalized to β-actin. Data are expressed as means ± SEM (n = 3). *P < 0.05 relative to the corresponding normal group (Dunnett's t-test).