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. 2018 Aug 8;145(15):dev163386. doi: 10.1242/dev.163386

Fig. 6.

Fig. 6.

HDAC1 activity promotes pluripotency gene expression at the expense of lineage restriction. (A) Western blot analysis of lysates of aging animal pole explants from control embryos or embryos injected with HDAC1 mRNA examining H3K9Ac and H3K27Ac alongside total H3 and actin levels. Explants were grown alongside sibling embryos until late blastula (stage 9) and neural plate (stage 13) stages. (B,C) In situ hybridization examining epk and sox3 expression in aging animal pole explants from control embryos or embryos injected with HDAC1 mRNA. Explants were cultured alongside sibling embryos until late blastula (stage 9), mid-gastrula (stage 11) and neural plate (stage 13) stages. (D) qRT-PCR of explants from control embryos or embryos injected with HDAC1 mRNA examining expression of pluripotency genes (*P<0.05, ***P<0.005). Explants were cultured alongside sibling embryos until the neural plate stage (stage 13). Scale bars: 250 μm.