Fig. 7.

HDAC activity promotes neural crest formation. (A,B) In situ hybridization (A) and qRT-PCR (B) examining snail2 and foxd3 expression in animal cap explants induced with levels of Pax3/Zic1 titrated for weak neural crest establishment, with/without co-expression of HDAC1 (*P<0.05, ***P<0.005). Explants were cultured with sibling embryos until late neurula stages (stage 18). (C,D) Western blot analysis of lysates of control explants and explants induced with Pax3/Zic1 examining H3K9Ac and H3K27Ac alongside total H3 levels via chemiluminescence (C) and quantified using Odyssey (D) (*P<0.05, **P<0.01). Explants were cultured alongside sibling embryos until late blastula (stage 9) and neural plate (stage 13) stages. Scale bars: 250 μm.