Table 1. Primers used in this study.

Gene	Sequence (5′ → 3′)	Size (bp)	Description	Reference
chuA	GAC GAA CCA ACG GTC AGG AT	279	Hemetransport in Enterohemorrhagic O157:H7 E.coli	Clermont, Bonacorsi & Bingen (2000)
	TGC CGC CAG TAC CAA AGA CA
yjaA	TGA AGT GTC AGG AGA CGC TG	211	Protein of function unknown	Clermont, Bonacorsi & Bingen (2000)
	ATG GAG AAT GCG TTC CTC AAC
TSPE4.C2	GAG TAA TGT CGG GGC ATT CA	152	Putative DNA fragment (TSPE4.C2) in E. coli	Clermont, Bonacorsi & Bingen (2000)
	CGC GCC AAC AAA GTA TTA CG
hlyA	AAC AAG GAT AAG CAC TGT TCT GGC	1,777	Hemolysin	Koga et al. (2014)
	ACC ATA TAA GCG GTC ATT CCC GTC
cnf1	AAGATGGAG TTT CCT ATGCAGGAG	498	Cytotoxic necrotizing factor 1	Johnson & Stell (2000)
	CAT TCA GAG TCC TGC CCT CAT TAT T
iutA	GGC TGG ACA TCA TGG GAA CTG G	302	Aerobactin siderophore receptor	Koga et al. (2014)
	CGT CGG GAA CGG GTA GAA TCG
fyuA	TGA TTA ACC CCG CGA CGG AA	880	Yersiniabactin	Johnson & Stell (2000)
	CGC AGT AGG CAC GAT CTT GTA
iroN	AAT CCG GCA AAG AGA CGA ACC GCC T	553	Salmochelin siderophore receptor	Koga et al. (2014)
	GTT CGG GCA ACC CCT GCT TTG ACT TT
papG	CTG TAA TTA CGG AAG TGA TTT CTG	1,070	P fimbriae	Johnson & Stell (2000)
	ACT ATC CGG CTC CGG ATA AAC CAT
sfaS	GTG GAT ACG ACG ATT ACT GTG	240	Sfa fimbriae	Johnson & Stell (2000)
	CCG CCA GCA TTC CCT GTA TTC
iss	CAG CAA CCC GAA CCA CTT GAT G	323	Episomal increased serum survival	Johnson et al. (2008)
	AGC ATT GCC AGA GCG GCA GAA
traT	GGT GTG GTG CGA TGA GCA CAG	290	Serum resistance	Johnson & Stell (2000)
	CAC GGT TCA GCC ATC CCT GAG
ibeA	AGG CAG GTG TGC GCC GCG TAC	170	Invasion of brain endothelium	Johnson & Stell (2000)
	TGG TGC TCC GGC AAA CCA TGC
kpsMT II	GCGCAT TTGCTGATA CTGTTG	272	Capsule synthesis K1 e K5	Johnson & Stell (2000)
	CAT CCA GAC GAT AAG CAT GAG CA
ompT	TCA TCC CGG AAG CCT CCC TCA CTA CTA T	496	Episomal outer membrane protease	Koga et al. (2014)
	TAG CGT TTG CTG CAC TGG CTT CTG ATA C
rfb	TACCGACGACGCCGATCTG	300	O-typing O25b serotype	Clermont et al. (2008)
	TGCTATTCATTATGCGCAGC
PAI	GGACATCCTGTTACAGCGCGCA	930	pathogenicity-associated island (PAI) marker	Johnson & Stell (2000)
	TCGCCACCAATCACAGCCGAAC
adk	ATTCTGCTTGGCGCTCCGGG	583	adenylate kinase	Maluta et al. (2014), Wirth et al. (2006)
	CCGTCAACTTTCGCGTATTT
fumC	TCACAGGTCGCCAGCGCTTC	806	fumarate hydratase	Maluta et al. (2014), Wirth et al. (2006)
	GTACGCAGCGAAAAAGATTC
gryB	TCGGCGACACGGATGACGGC	911	DNA gyrase	Maluta et al. (2014), Wirth et al. (2006)
	ATCAGGCCTTCACGCGCATC
icd	ATGGAAAGTAAAGTAGTTGTT CCGGCACA	878	isocitrate/isopropylmalate dehydrogenase	Maluta et al. (2014), Wirth et al. (2006)
	GGACGCAGCAGGATCTGTT
mdh	ATGAAAGTCGCAGTCCTCGGCGCT GCTGGCGG	932	malate dehydrogenase	Maluta et al. (2014), Wirth et al. (2006)
	TTAACGAACTCCTGCCCCAGAGCG ATATCTTTCTT
purA	CGCGCTGATGAAAGAGATGA	816	adenylosuccinate dehydrogenase	Maluta et al. (2014), Wirth et al. (2006)
	CATACGGTAAGCCACGCAGA
recA	CGCATTCGCTTTACCCTGACC	780	ATP/GTP binding motif	Maluta et al. (2014), Wirth et al. (2006)
	AGCGTGAAGGTAAAACCTGTG
16S rRNA	AGAGTTTGATCCTGGCTCAG	1,465	16S rRNA, partial gene	Suardana (2014)
	GGTTACCTTGTTACGACTT