Skip to main content
. 2018 Aug 24;6:e5503. doi: 10.7717/peerj.5503

Figure 4. The difference between linear RNA and circRNA primer design.

Figure 4

(A) FW is a forward primer with the b chain as template. The base sequence of synthesis is the original sequence of a. RV is a reverse primer with a chain as template, and the base sequence of synthesis is the original sequence of b. The sequence between FW and RV is high; (B) The original primers need to reverse: the synthetic primers are FW′ and RV′, where FW′ is the reverse complementary sequence of the RV primer, RV′ is the reverse complementary sequence of FW primer.