Figure 2. Knockdown of antisense non-coding RNA in the INK4 locus (ANRIL) inhibited cell viability, migration, and invasion, and promoted apoptosis. Untreated cells acted as control. A, Transfection efficiency in MKN-45 and SGC-7901 cells tested by qPCR. GAPDH acted as an internal control. B, Rate of apoptotic cells detected by flow cytometry. C and D, Cell viability determined by CCK-8 assay. The migration (E) and invasion (F) were measured by Transwell assay. shANRIL: pENTRTM/U6 vector carrying small hairpin RNA targeting ANRIL; shNC: pENTRTM/U6 vector carrying a non-targeting sequence; qPCR, quantitative real-time PCR; CCK-8, Cell Counting Kit-8. Data are reported as means±SD.*P<0.05; **P<0.01; ***P<0.001 (two-tailed Student's t-test).
