Figure 5. Knockdown of antisense non-coding RNA in the INK4 locus (ANRIL) inhibited the expression of BMI1 via miR-99a up-regulation. Protein expressions of BMI1 were measured by western blotting. A, ANRIL silence down-regulated BMI1 expression. B, Expression of BMI1 was negatively correlated with miR-99a expression. C, Putative binding sequence between miR-99a-3p and BMI1 3′UTR using TargetScan software. D, Down-regulation of BMI1, induced by ANRIL knockdown, was reversed by miR-99a inhibition. shANRIL: pENTRTM/U6 vector carrying small hairpin RNA targeting ANRIL; shNC: pENTRTM/U6 vector carrying a non-targeting sequence; miR-99a: microRNA-99a; BMI1: B-lymphoma Mo-MLV insertion region 1; 3′UTR: 3′-untranslated region.
