Fig 1. AminoLink resin protocol.

Molecular view of the immobilization step (A) and overall view of the role protocol (B) AminoLink resins contain aldehyde groups that reacted with the free amino groups of TSC or TSC-C. The aldehyde sites that do not react with the compound are blocked with sodium cyanoborohydride (A). After compound immobilization, the fungal protein extract is added to the resin column. The proteins that interact with the compounds stay in the resin and the rest of the proteins are washed away. The interacting proteins are eluted from the resin, digested, and identified through mass spectrometry (B).