Fig 3. Effect of incubation with PLA2G1B (20 μg/mL) on IL-8 secretion from bovine mammary epithelial cells treated with LPS, Escherichia coli or Staphylococcus aureus.

Cells were seeded in 48-well culture plates and examined at three time points post-treatment; levels of IL-8 in the growth medium were calculated by ELISA test. Standard error bars are presented (n = 6). Cells were first treated with the inflammation agent for 4 h and then incubated in fresh medium with PLA2G1B. Examination time points were 1, 2 and 3 days. Asterisks denote significant differences among the three groups of treatment types denoted by each of the horizontal bars (P < 0.05).