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. 2018 Jul 30;10(8):2110–2129. doi: 10.1093/gbe/evy157

Fig. 3.

Fig. 3.

—Identification of the W chromosome and sex-chromosome-linked genes in Thamnophis sirtalis. (A) Maximum likelihood trees of fragments of six genes with Z and W alleles in T. sirtalis. Circles at nodes represent bootstrap values: Black circles indicate bootstrap values >70; white circles indicate bootstrap values <70. (B) Female-specific amplification of PCR primers designed from T. sirtalis W scaffolds in two male and two female T. sirtalis samples for six genes. Primer sequences are in supplementary table S5, Supplementary Material online. (C) Fluorescent in situ hybridization (FISH) of a Bkm-like repeat, (GATA)n in a female T. sirtalis. Sex-specific hybridization on the W chromosome is indicated by an arrow.