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. 2018 Jul 17;17(10):1245–1254. doi: 10.1080/15384101.2018.1471317

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Figure 3. — Upregulation of ZEB1-AS1 increases CRC cell proliferation. (a) RT–qPCR analysis of ZEB1-AS1 expression in RKO and HCT116 cells transfected with pcDNA3.1-ZEB1-AS1 or empty pcDNA3.1 vector (mean ± SD, n = 6; *P < 0.05 vs pcDNA3.1). (b) Upregulation of ZEB1-AS1 promoted proliferation in RKO and HCT116 cells, as revealed by CellTiter 96® AQueous One Solution Cell Proliferation assay (n = 6; *P < 0.05 vs pcDNA3.1). (c) Histological analysis of the rates of colony formation in pcDNA3.1 and ZEB1-AS1-overexpressing groups (n = 6; *P < 0.05 vs pcDNA3.1). (d-e) EdU incorporation assays were used to examine the effects of ZEB1-AS1 overexpression on DNA synthesis during cell growth. Images were taken at × 200. (n = 6; *P < 0.05 vs pcDNA3.1). (f-g) Western blotting showed that the proportion of S-phase cells (EdU-positive cells) was increased in pcDNA3.1-ZEB1-AS1-treated groups (n = 6, *P < 0.05 vs pcDNA3.1).