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. 2018 Jul 17;17(10):1245–1254. doi: 10.1080/15384101.2018.1471317

Figure 4.

Figure 4.

Negative regulation of ZEB1-AS1 by miR-181a-5p. (a) Predicted miR-181a-5p binding sites in the ZEB1-AS1 sequence. Numbers indicate nucleotide positions relative to the ZEB1-AS1 transcriptional start site. (b) miR-181a-5p mRNA levels were plotted against ZEB1-AS1 expression in 65 CRC specimens, demonstrating a significant positive correlation (two-tailed Pearson’s correlation, r = 0.706; P < 0.01). (c, d) RKO and LOVO cells were transfected with a miR-181a-5p inhibitor (c) or mimic (d), and ZEB1-AS1 expression was analyzed by RT-qPCR 48 h later (n = 6, *P < 0.05 vs NC). (e-f) RT–qPCR was used to detect ZEB1-AS1, miR-181a-5p, GAPDH and MALAT1 levels in the cytoplasmic and nuclear fractions of RKO and LOVO cells. GAPDH and MALAT1 served as a cytoplasmic and nuclear localization marker, respectively (n = 6, *P < 0.05 vs NC).