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. 2018 Jul 17;17(10):1245–1254. doi: 10.1080/15384101.2018.1471317

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Figure 5. — MiR-181a-5p inhibits ZEB1-AS1 function. (a) RKO and LOVO cells were co-transfected with a negative control or miR-181a-5p mimic and either a control plasmid (pcDNA3.1) or ZEB1-AS1 expression plasmid (pcDNA3.1-ZEB1-AS1). Cell viability was determined using CellTiter 96® AQueous One Solution Cell Proliferation assays (n = 6; *P < 0.05 vs NC+pcDNA3.1; #P < 0.05 vs NC+pcDNA3.1-ZEB1-AS1). (b-c) Colony-formation assay in CRC cells after cotransfection with a negative control or miR-181a-5p mimic and a control plasmid or ZEB1-AS1 expression plasmid (n = 6; *P < 0.05 vs NC+pcDNA3.1; #P < 0.05 vs NC+pcDNA3.1-ZEB1-AS1).