Figure 6.

Evaluation of lifespan-mediated changes in nucleolus in haploid wild-type yeast strain BY4741, isogenic mutant strains lcl1Δ, lcl2Δ and bud1Δ (rho⁺ and rho°) and clones generated as daughters of an old mother. (a) Analysis of rDNA content. rDNA was visualised using WCPP specific to chromosome XII that contains rDNA locus in yeast. Fluorescence signals of chromosome XII were quantified using the ImageJ software. The integrated fluorescence density is presented in relative fluorescence units (RFUs). Box-and-whisker plots are shown, n = 100. The typical micrographs are shown (bottom). *p < 0.05, **p < 0.01, ***p < 0.001compared to the rho⁺ strain, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 compared to the rho° strain (ANOVA and Dunnett’s a posteriori test). The cells were labelled with FITC to detect chromosome XII-specific signals (green). DNA was visualised using DAPI staining (blue). (b) Silver staining of nucleolar organiser region-based analysis of nucleolus fragmentation. Fragmented nucleoli were scored (%). The typical micrographs are also shown (bottom).