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. 2018 Jul 17;17(10):1255–1267. doi: 10.1080/15384101.2018.1471318

Figure 2.

Figure 2.

Effects of melatonin exposure on gsHFSCs proliferation and single hair follicle morphological changes. (A) Representative images depicting gsHFSCs morphology after exposure to different concentrations of melatonin at 24h. Scale bar, 200 μm. (B) Cell viability analysis of different concentrations of melatonin on gsHFSCs proliferation following 24 and 36 h exposure. (C) Flow cytometry and immunofluorescence analysis of EdU positive gsHFSCs after 0 and 500 ng/L melatonin exposure at 36 h. (D) Scheme diagram for in vitro 3D agar-based goat single hair follicle culture system. (E) Microscopic comparison of hair shafts between the control and 500 ng/L melatonin exposed Cashmere goat secondary hair follicles. Cashmere goat hair follicles were culture in agar based 3-D culture system for a week. Scale bar, 40 μm. (F) TEM pictures of Cashmere goat secondary hair follicle dermal papilla after melatonin exposure. Scale bar, 2 μm. (G) TEM pictures of Cashmere goat secondary hair follicle outer root sheath. Scale bar, 2 μm.