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. 2018 Jul 18;17(9):1138–1145. doi: 10.1080/15384101.2018.1464836

Figure 1.

Figure 1.

Inhibition of Cdh1 suppresses NHEJ in G1 cells. (A) Quantification of NHEJ assay in cells transfected with indicated siRNAs. U2OS cells were transfected with indicated siRNAs and treated with lovastatin. The NHEJ efficiency was normalized to that in control (siCTL) cells. (B) Survival of U2OS cells transfected with the indicated siRNAs after irradiation. Twenty-four hours after transfection of the siRNAs, the cells were treated with lovastatin for 36 h and then cultured in fresh media for 2 weeks after indicated doses of irradiation. (C) Cell cycle analysis of the cells transfected with indicated siRNAs. U2OS cells were transfected with indicated siRNAs and next day cells were treated with lovastatin for 36 h and then irradiated. Cell cycle distribution was measured at the indicated time points after release from lovastatin arrest and IR. Error bars indicate SEM from three independent experiments. ** (in A) indicates P < 0.001 (student’s t-test).