Figure 3.

Cytobiology change of human 293T and SH-SY5Y cell lines transfected with circ-PRKCI siRNA.

(a) Knockdown of circ-PRKCI suppressed cell proliferation, while overexpression of circ-PRKCI promoted the cell proliferation. Absorbance at 450 nm measured by CCK8 was expressed as Mean ± SE (left panel). The EDU assay showed the representative images of proliferated cells (middle panel). Quantification of cell proliferation were presented as percentage proliferated cell numbers (right panel). (b) Transwell assay was performed as described in materials and methods. The quantifications of cell migration were presented as percentage migrated cell numbers, which indicated that down-regulation of circ-PRKCI inhibited cell migration and overexpression of circ-PRKCI had the opposite effect.