Figure 5.

Circ-PRKCI regulated cell proliferation and migration via the miR-1324-PLCB1 axis.

(a, b) qRT-PCR and western blot analysis of PLCB1 were conducted in human 293T and SH-SY5Y cell lines after transfection with circ-PRKCI siRNA with or without miR-1324 inhibitor. GAPDH was used as control. (c, d) Two types of cells were transfected with miR-1324 mimics with or without circ-PRKCI overexpress plasmid. The mRNA level of PLCB1 and relative PLCB1 protein level were detected. (e, f) CCK8 assay and EDU assay were performed to determine the proliferation of cells transfected with miR-1324 mimics and cells treated with miR-1324 mimics in combination with circ-PRKCI overexpress plasmid. (g) Transwell analysis of 293T and SH-SY5Y cells transfected with miR-1324 with or without circ-PRKCI overexpress plasmid (left panel); Migrated cells stained with crystal violet was shown (right panel).