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. 2018 Jul 17;17(9):1124–1137. doi: 10.1080/15384101.2018.1480218

Figure 3.

Figure 3.

PI3K inhibition reduces SAMHD1 KO-induced proliferation in THP-1 cells but does not affect apoptosis. (a) THP-1 control (Ctrl) and SAMHD1 KO cells were treated with DMSO or the PI3K inhibitor LY294002 at 5 µM. Cells were analyzed by an MTT-based cell proliferation assay at the indicated time points. One representative experiment performed in three replicates is shown. OD, optical density at 490 nm. *, P < 0.01; **, P < 0.001; ***, P ≤ 0.0001. (b-c) THP-1 control (Ctrl) or SAMHD1 KO cells were treated with DMSO or 25 µM LY294002 for 18 h and were analyzed via (b) annexin-V/7-AAD apoptosis assay and (c) via caspase-glo 3/7 activity assay to determine the apoptosis levels and caspase activities, respectively. Each experiment was performed in three biological replicates.