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. 2018 Jul 17;17(9):1124–1137. doi: 10.1080/15384101.2018.1480218

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Figure 4. — SAMHD1 KO inhibits nuclear localization of p27 in THP-1 cells. (a) THP-1 control and SAMHD1 KO cells were analyzed via subcellular fractionation followed by immunoblotting. GAPDH and Lamin A/C were used as markers for cytosolic and nuclear fraction, respectively. Results are representative of three independent experiments. (b) Cells were analyzed via indirect immunofluorescence for p27 (red) and SAMHD1 (green) expression. DAPI (blue) was used to stain the nuclei. Scale bars, 15 µm. Results are from one representative experiment of two independent assays.