Figure 7.

SAMHD1 KO attenuates THP-1 cell tumorigenicity in xenograft mice. Female, 4–6-week old NSG mice (n = 7 per group) were subcutaneously injected with THP-1 control or THP-1/KO cells (5 × 10⁶ per mouse). (a) The rate of tumor growth and tumor volume were assessed at the time indicated. Mice were sacrificed when the tumors reached a volume of 1,600 mm³. (b) Average volume of subcutaneous tumors from the mice of both groups was calculated at 24 days post-injection (6 out of 7 mice developed measurable tumors). (c) Upon sacrificing the mice, tumor tissues were collected and harvested proteins were analyzed via immunoblotting for TNF-α and SAMHD1. GAPDH was a loading control. (d) Densitometry analysis was performed to quantify the relative TNF-α protein levels in control and THP-1/KO cell-derived tumors. The results were based on the normalization to GAPDH levels. (e) Pearson’s correlation analysis was performed to determine correlation between subcutaneous tumor volume and the TNF-α protein levels. All results are representative of two independent experiments. (b, d and e) Statistical significance was determined by unpaired Students t-test and the P values are shown on the figures.