Figure 7.

(A) Phosphorylation of ChREBP by PKA and effects of PKA on the DNA-binding activity of ChREBP in vitro. Recombinant C-terminal ChREBP was incubated with PKA in the presence of [γ-³²P]ATP. At given time intervals, [³²P]phosphate incorporation (□) and DNA-binding activity (■) were determined as described in Experimental Procedures. The values are expressed as mean ± SEM. (Insert) Representative image for effects of PKA on the DNA-binding activity of ChREBP using gel shift assay. (B) Dephosphorylation of ChREBP by PP2A and the effect of PP2A on the DNA-binding activity of ChREBP in vitro. Phosphorylated C-terminal ChREBP was incubated with PP2A. At given time intervals, remaining phosphorylated C-terminal ChREBP (□) and DNA-binding activity (■) were determined as described in Experimental Procedures. The values are expressed as mean ± SEM. (Insert) Representative image for effects of PP2A on the DNA-binding activity of ChREBP.