Table 2.

Oligonucleotides used to construct plasmids containing point mutations in the putative phosphorylation sites of PKA

Mutant	Orientation	Sequence (position)
S196A	Forward	5′-CTCCGTAAGTCCGCCAGGGAAGGGGAT-3′ (bp 574–600)
	Reverse	5′-ATCCCCTTCCCTGGCGGACTTACGGAG-3′ (bp 574–600)
S196D	Forward	5′-CTCCGTAAGTCCGACAGGGAAGGGGAT-3′ (bp 574–600)
	Reverse	5′-ATCCCCTTCCCTGTCGGACTTACGGAG-3′ (bp 574–600)
S626A	Forward	5′-GAGCGGCGACTAGCCGGGGATCTCAAC-3′ (bp 1864–1890)
	Reverse	5′-GTTGAGATCCCCGGCTAGTCGCCGCTC-3′ (bp 1864–1890)
S626D	Forward	5′-GAGCGGCGACTAGACGGGGATCTCAAC-3′ (bp 1864–1890)
	Reverse	5′-GTTGAGATCCCCGTCTAGTCGCCGCTC-3′ (bp 1864–1890)
T666A	Forward	5′-AACCGACGTATCGCCCACATCTCCGCG-3′ (bp 1981–2007)
	Reverse	5′-CGCGGAGATGTGGGCGATACGTCGGTT-3′ (bp 1981–2007)
T666D	Forward	5′-AACCGACGTATCGACCACATCTCCGCG-3′ (bp 1981–2007)
	Reverse	5′-CGCGGAGATGTGGTCGATACGTCGGTT-3′ (bp 1981–2007)

The mutated nucleotides are underlined.