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. 2018 Jul 9;17(9):1115–1123. doi: 10.1080/15384101.2018.1480217

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Figure 4. — Ionizing radiation increased the activity of the serum response factor (SRF) by promoting the nuclear accumulation of megakaryocytic acute leukemia (MAL) factor.

(a) A graphical representation of the plasmid construction to determine the SRF activity. (b) The intracellular protein hybridization of MAL and SRF. (c) The percentage of MAL in the nucleus. (d) Results of a Co-IP experiment using MAL antibody as the immunoprecipitating antibody to confirm interactions between MAL and its binding partners SRF. Normal rabbit IgG was used for Co-IP and served as a negative control, whereas normal cells lysate without Co-IP served as a positive control. The heavy chain of IgG served as a loading control. (e) The SRF activity was determined using the luciferase reporter assay. The error bars denote the mean± SE derived from three independent experiments.