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. 2018 Jul 5;17(10):1188–1198. doi: 10.1080/15384101.2018.1467675

Figure 6.

Figure 6.

LSINCT5 physically interacts with HMGA2 to inhibit its degradation. (A) Relative HMGA2 mRNA levels with si-LSINCT5 transfection in 95D cells or pcDNA-LSINCT5 transfection in 95C cells. (B) Immunoblots for HMGA2 protein expression by si-LSINCT5 in 95D cells or pcDNA-LSINCT5 transfection in 95C cells. (C) 95D cells were transfected with si-NC or si-LSINCT5 and then treated with MG132 (30 μM) for 12 h followed by immunoblot for HMGA2. (D) A549 cells were transfected with si-NC or si-LSINCT5 and then treated with MG132 (30 μM) for 12 h followed by immunoblot for HMGA2. (E) Cell lysates from 95D cells with si-LSINCT5 or 95C cells with LSINCT5 overexpression were immunoprecipitated with HMGA2 and then subject to immunoblot towards ubiquitin or HMGA2. (F) Migration in 95D cells transfected with pcDNA, si-NC, si-HMGA2 (#2), pcDNA-LSINCT5 or pcDNA-LSINCT5 plus si-HMGA2. **: P < 0.01. (G) Rescue transwell migration assays were done in 95C cells. LSINCT5 were silenced or not and 95C cells were transfected with either pcDNA or pcDNA-HMGA2. **: P < 0.01.