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. 2018 Aug 21;11:5075–5082. doi: 10.2147/OTT.S161316

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© 2018 Xu et al. This work is published and licensed by Dove Medical Press Limited

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Expression of miR-543 and SPOP in GC cells. (A) qPCR analysis was performed to detect the expression of miR-543 in MKN45 and AGS cells transfected with NC oligonucleotide (CON) or miR-543 inhibitor. (B and C) qPCR and western blot analysis showed that miR-543 knockdown upregulated the mRNA and protein level of SPOP in MKN45 and AGS cells. Cells were transfected with the miR-543 inhibitor or NC. *P<0.05, compared with CON.

Abbreviations: GC, gastric cancer; miR-543, microRNA-543; NC, control; qPCR, quantitative polymerase chain reaction; SPOP, speckle-type POZ protein.