Figure 4.
Rescue of a fringe effect with β4GalT-1. (A) Lectin sensitivity test. Cells surviving in E-phytohemagglutinin (PHA) were stained with methylene blue. (B) Gel filtration profiles of [3H]fucose-labeled O-glycans released by β-elimination from Notch1 EGF19–23 produced by Lec20 cells expressing Lfng and β4GalT-1. Elution positions of O-fucose glycan standards are indicated. (C) Fold-activation of normalized luciferase units in Lec2 or Lec20 clones expressing vector, Lfng, or Lfng + GT (β4GalT-1) stimulated by J1 vs. L cells. Error bars represent SEM; n = 4.