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. 2018 Aug 27;8:12827. doi: 10.1038/s41598-018-30072-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Determination of distribution and intensity of NP, ANP, INP and AINP in the rat brain tissues by fluorescence imaging after treatment with NP, ANP, INP and AINP. (A) Fluorescence images, endothelial cells are stained in blue, the neuroglial cells are stained in red and green color represents the NPs; (B) ex vivo fluorescence imaging of brain separated from BALB/c nude mice 0.5 h, 2 h and 4 h after intravenous administration of NP, ANP, INP and AINP. (C) RhoB-Labeled NPs, ANPs, AINPs, and INPs ingested by BCECs.