Figure 4.

Dimerization correlates with effective anti-inflammatory activity in vitro. A549 GRE-luc cells were stimulated with the compounds (10⁻⁸ M) for 1 h, followed by challenge with 1000 U/ml hTNF. After 24 h, hIL6 levels were measured in the supernatant. Data are shown as mean ± SEM. A Hierarchical Generalized Linear Mixed Model (HGLMM) was fitted to the expression data. T statistics (unpaired, two-tailed) were used to assess the significance of fixed compound effects estimated as differences (on the transformed scale) to NI (significances above error bars), TNF (indicated with−) and Dex (indicated with dotted−) set as reference. Estimated mean values were obtained as predictions from the HGLMM, formed on the scale of the response variable. P-values < 0.05, 0.01, 0.001 and 0.0001 are represented by *, **, *** or **** respectively. Non-significant differences are not indicated on the graph. The experiment was repeated twice with at least three technical replicates included in the individual experiments. NI: Non-Induced, TNF: Tumor Necrosis Factor, Dex: Dexamethasone.