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. 2018 Aug 27;8:12828. doi: 10.1038/s41598-018-31200-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Primer and probe layout on the TAC card. A total of 27 primer/probe pairs were printed on the TAC. MS2 and PhHV were used as internal positive controls. Human 18S rRNA (ABI quality control) was used as a quality control for the TAC card. The 27 primer/probe pairs included Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Staphylococcus hominis (S. hominis), Staphylococcus haemolyticus (S. haemolyticus), Klebsiella oxytoca (K. oxytoca), Enterococcus faecium (E. faecium), Enterococcus faecalis (E. faecalis), Streptococcus pneumoniae (S. pneumoniae), Streptococcus agalactiae (S. agalactiae), Streptococcus pyogenes (S. pyogenes), Clostridium perfringens (C. perfringens), Serratia marcescens (S. marcescens), Listeria monocytogenes (L. monocytogenes), Escherichia coli (E. coli), Klebsiella pneumoniae (K. pneumoniae), Acinetobacter baumannii (Ab), Stenotrophomonas maltophilia (S. maltophilia), Pseudomonas aeruginosa (P. aeruginosa), Achromobacter xylosoxidans (A. xylosoxidans), Burkholderia cepacia (B. cepacia), Neisseria meningitides (N. meningitides), Haemophilus influenza (H. influenzae), Cryptococcus neoformans (C. neoformans),Candida glabrata (C. glabrata), Candida tropicalis (C. tropicalis), Candida albicans (C. albicans), Candida parapsilosis (C. parapsilosis).