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. 2018 Aug 27;8:12875. doi: 10.1038/s41598-018-30659-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of neurotrophic (BDNF) and anti-apoptotic (Bcl-xL) factors expression levels. Western blot assay was performed in the cytoplasmic fraction of the lumbar spinal cord of WT mice and VEH, LD and HD SOD1(G93A) groups (n = 5 per group). (a) Representative western blot image depicting the expression of BDNF (14 kDa), Bcl-xL (30 kDa) and β-actin (45 kDa). (b–c) Densitometric analysis of the expression of BDNF and Bcl-xL normalized to β-actin. In (b) note the significantly decreased expression of BDNF in VEH group compared to WT (WT vs VEH, ^#P = 0.0280) and the increase of BDNF expression after drugs treatment, statistically significant in HD group (VEH vs HD, ^*P = 0.0206). In (c) note the significant increase of Bcl-xL expression after the treatment with the high dose of drugs (VEH vs HD ^**P = 0.0054). All gels and blots were processed in parallel showing similar results. Results were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. Graphs are shown as mean ± SEM. ^#indicates p < 0.05 of interested group vs WT group; ^* and ^**indicate p < 0.05 and p < 0.01 respectively of the interested group vs VEH group. Full length blots are shown in Supplementary Information, in Supplemental Fig. 3.